We used sera from 8 naturally infected horses which had recovered from Italian outbreaks and 2 experimentally infected mares. Development of the cIB assay was based on the hypothesis that serum IgGs from healthy and infected animals recognise different T. One compounding factor is that sera from uninfected animals often cross-react with T. humoral response following the infection. In this study we developed a chemiluminescent immunoblotting (cIB) assay to characterise the Trypanosoma equiperdum antigen pattern recognised by IgGs from naturally or experimentally dourine-infected horses and analyse the kinetics of IgG. Read moreĭiagnosis and control of dourine is strongly based on serological evidence, but knowledge of the humoral response of horses during infection is limited. BLAST analyses of this epitope against all Enterovirus entries in Genbank also demonstrated that this epitope is unique in EV71, but not other Enterovirus such as CA16 It may be useful for structural study of VP1 morphogenesis during infection and also applications for identification of EV71 infection. mAb 4 can cross-react with all the 11 representative EV71 subgenotypes (A, B1-5, C1-5), but not with the representative strain of CA16 as demonstrated by immunofluoresence assay (IFA). antibody (mAb 4) specific for EV71 was generated and mapped to target the N-terminal region of VP1 capsid protein, spanning amino acid residues 12-19 (IGDSVSRA). Lots of attention has been placed on elucidating viral epitopes, which is useful for EV71 viral research. Hand, foot and mouth disease (HFMD) is a viral infectious disease caused by human Enterovirus A, particularly Enterovirus 71 (EV71) and Coxsackievirus 16 (CA16) serotypes, with EV71 infection associated with severe neurological complications and mortality.

Italian explain ifa odus